Re: LEDs

Posted by Emmanuel Gustin on
URL: http://confocal-microscopy-list.275.s1.nabble.com/LEDs-tp4288043p4290676.html

CONFOCALMICROSCOPY Digest - 8 Jan 2010 to 10 Jan 2010 (#2010-4)

Hi,

 

I agree with Rich’s list of advantages, but of course there are also some limitations to take into account, at least in my limited experience.

 

Directly coupled LEDs don’t have the same illumination homogeneity as the latest generation of liquid light guide-coupled lamps. If you don’t adjust the condensor lens correctly, you can see the structure of the LED in your image. Of course you also have that problem with a directly attached lamp, and I guess it would be eliminated by using a light guide with a LEDs.

 

LEDS are not strictly monochromatic but have emission bands composed of a set of transitions. When I was a student, one exercise we were asked to do was to dip LEDs in liquid nitrogen, to get narrower bands, and analyze their spectra and to figure out what semiconductors and doping had been used in their construction... Unlike when you use a semiconductor laser, you may still need to use excitation filters with a LED.

 

best regards,

 

Emmanuel