Re: Live cell imaging experiences, please

Posted by Neeraj Gohad-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Live-cell-imaging-experiences-please-tp4285373p4292521.html

Hi Zoltan,

I have done long term time lapse (24 hour) experiments looking at multiple stage points in 3 different channels. We have a Nikon TiE with a motorized stage, perfect focus system and a Sutter Lambda 10-3 mechanical shutter. Its fairly easy to setup multidimensional experiments in the Nikon elements software, you can setup pretty complicated configurations which can be saved and reloaded. Using the perfect focus you can set different Z offsets for different stage points, there is also a function which automatically optimizes the stage travel between the stage points.


As always, no commercial interests just a satisfied customer.


Neeraj V. Gohad, Ph.D.
Postdoctoral Fellow
Okeanos Research Group
Department of Biological Sciences
132 Long Hall
Clemson University
Clemson,SC-29634

Please note my new email address: [hidden email]


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Christian Götze
Sent: Tuesday, January 12, 2010 5:36 AM
To: [hidden email]
Subject: Re: Live cell imaging experiences, please

Commercial response!

Hi Zoltan,

exciting application! Please correct me, if I'm wrong but one of these
experiments would result in about 12.5 GByte of data (at 512x512 Pixels
per plane, 8 Bit intensity, 18 Planes per stack, 8 stacks per time
point, 1800 time points). What kind of software do you plan to use?

If you don't have a suitable candidate already, perhaps you should have
a look on our arivis Browser software. It is specialized in handling
very large experiments and could be the right solution for you.

(http://www.arivis.com/en/Produkte/arivis-Browser)

If you are interested or there are questions please don't hestitate to
contact me.

Christian

Zoltan Cseresnyes schrieb:

> Dear All,
>
> We are planning to run a few tests, where we follow cell development
> in 8-9 XY positions, running 45 um Z stacks (step size 2.5 um), 2.5
> min time interval, for a total of 14 hours at room temp, using GFP and
> DsRed settings.  We had lots of success with the Leica SP5 system for
> the past 2 years.  We are wondering if this community has more input
> about how other systems would perfom here?  We are most interested in
> Olympus FV1000 and Nikon A1.  Our main concerns are the simplicity of
> the setting up procedure, the stability of the system during the long
> scans, and the handling of the large data files.
>   All inputs will be much appreciated!  Happy New Year to all!  With
> best wishes,
>
> Zoltan
>

--
Christian Götze

arivis - Multiple Image Tools GmbH
Kröpeliner Straße 54, D-18055 Rostock

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