Solid Tumors in Living Color
The behavior of tumors is profoundly influenced
by the microenvironment in which they grow. In
addition to diffusible extracellular factors, this
environment harbors a complex and dynamic
population of stromal cells, including fibroblasts
and a variety of immune cells. Because different
types of stromal cells can have opposing effects
on tumor progression and responses to therapy,
it is important to understand how each cell type
behaves in actively growing tumors.
Egeblad et al. have combined confocal
microscopy with multicolor imaging techniques
to record in living mice the movement and localization
patterns of tumor-infiltrating stromal
cells during a 12-hour period. One feature
shared by several stromal cell types was greater
motility at the tumor periphery than within the
tumor mass. Regulatory T cells were found to
migrate near blood vessels, and their movement
was sensitive to tumor oxygen levels; in contrast,
the movement of myeloid cells (the most heterogeneous
group of stromal cells) was insensitive
to oxygen, and their localization patterns and
migration rates varied according to cell-surface
marker expression, probably reflecting important
functional differences. By helping to define
the contributions of specific stromal cells to
tumor growth, this imaging technology may lead
to more effective therapies. — PAK
Disease Models Mech. 1, 155 (2008).
George A. Peeters MD, MS
President, Solamere Technology Group Inc
1427 Perry Ave
Salt Lake City, UT 84103
www.solameretech.com
801 322-2645 office 801 322-2645 fax
801 232-6911 cell
On Jan 11, 2010, at 6:31 AM, Zoltan Cseresnyes wrote:
Dear All,
We are planning to run a few tests, where we follow cell development
in 8-9 XY positions, running 45 um Z stacks (step size 2.5 um), 2.5
min time interval, for a total of 14 hours at room temp, using GFP and
DsRed settings. We had lots of success with the Leica SP5 system for
the past 2 years. We are wondering if this community has more input
about how other systems would perfom here? We are most interested in
Olympus FV1000 and Nikon A1. Our main concerns are the simplicity of
the setting up procedure, the stability of the system during the long
scans, and the handling of the large data files.
All inputs will be much appreciated! Happy New Year to all! With
best wishes,
Zoltan
--
Zoltan Cseresnyes
Facility manager, Imaging Suite
Univ. Cambridge, UK