Re: Live cell imaging experiences, please

Posted by Adrian Smith-6 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Live-cell-imaging-experiences-please-tp4285373p4295773.html

On 12/01/2010, at 12:31 AM, Zoltan Cseresnyes wrote:

> Dear All,
>
> We are planning to run a few tests, where we follow cell development
> in 8-9 XY positions, running 45 um Z stacks (step size 2.5 um), 2.5
> min time interval, for a total of 14 hours at room temp, using GFP and
> DsRed settings.  We had lots of success with the Leica SP5 system for
> the past 2 years.  We are wondering if this community has more input
> about how other systems would perfom here?  We are most interested in
> Olympus FV1000 and Nikon A1.  Our main concerns are the simplicity of
> the setting up procedure, the stability of the system during the long
> scans, and the handling of the large data files.
> All inputs will be much appreciated!  Happy New Year to all!  With
> best wishes,
>
> Zoltan
>

Are you having particular problems with doing this on your SP5?

We've been doing overnight (and longer) multiposition z-stacks on our SP5 (with full enclosure) and it has been working very well.

Regards,

Adrian Smith
Centenary Institute, Sydney, Australia