Posted by
Richard Harris-6 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Live-cell-imaging-experiences-please-tp4285373p4394559.html
Hi Zoltan
We run a Zeiss Live 5 Duo (Vario 2) LSM system here and have had great
success collecting huge data sets on metamorphosis in drosophila - these are
typically 16 - 18 hour experiments collecting a Z-stack (20 or more slices)
every 2 1/2 minutes. The stage is very stable and we've been using the
stitching feature to generate large area, high resolution images - this
means we're collecting as many as 10 XY positions per critter every 2 - 3
minutes.
Results are impressive and we've been working at generating movies from
these HUGE 4D data sets.
The Zeiss system in Live mode is capable of generating up to 120 FPS (XY)
and/or fast Z-stacks (approx 8-10um/sec).
We are satisfied customers and have no commercial interest in Zeiss.
Rick,
Richard Harris, Manager - Integrated Microscopy @ Biotron
The Biotron - Center for Experimental Climate Change Research
University of Western Ontario,
London Ontario, CANADA.
N6A 5B7
Ph. 519-661-2111 ext. 86780
Fax 519-661-3935
e-mail
[hidden email]
web: www.thebiotron.ca
-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]] On
Behalf Of Zoltan Cseresnyes
Sent: Monday, January 11, 2010 8:31 AM
To:
[hidden email]
Subject: Live cell imaging experiences, please
Dear All,
We are planning to run a few tests, where we follow cell development
in 8-9 XY positions, running 45 um Z stacks (step size 2.5 um), 2.5
min time interval, for a total of 14 hours at room temp, using GFP and
DsRed settings. We had lots of success with the Leica SP5 system for
the past 2 years. We are wondering if this community has more input
about how other systems would perfom here? We are most interested in
Olympus FV1000 and Nikon A1. Our main concerns are the simplicity of
the setting up procedure, the stability of the system during the long
scans, and the handling of the large data files.
All inputs will be much appreciated! Happy New Year to all! With
best wishes,
Zoltan
--
Zoltan Cseresnyes
Facility manager, Imaging Suite
Univ. Cambridge, UK