Re: Live cell imaging experiences, please

Posted by Zoltan on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Live-cell-imaging-experiences-please-tp4285373p4423647.html

Thank you all very much for the very valuable feedback on the
long-term stability (hardware and software) of the FV1000 and A1
systems!  Just to clarify:  we have been using the SP5 systems of our
Imaging Facility for these scans for 2 years now and are happy with
them.  However, one of our users is now applying for a fellowship to
start his own lab outside the UK, and thus needed to get familiar with
the rest of the possible systems that are proven to be suitable for
such long scans (with large result files).
  Thank you all for your feedback again!  With best regards,

Zoltan


On Thu, Jan 14, 2010 at 6:48 PM, Richard Harris <[hidden email]> wrote:

> Hi Zoltan
> We run a Zeiss Live 5 Duo (Vario 2) LSM system here and have had great
> success collecting huge data sets on metamorphosis in drosophila - these are
> typically 16 - 18 hour experiments collecting a Z-stack (20 or more slices)
> every 2 1/2 minutes.  The stage is very stable and we've been using the
> stitching feature to generate large area, high resolution images - this
> means we're collecting as many as 10 XY positions per critter every 2 - 3
> minutes.
> Results are impressive and we've been working at generating movies from
> these HUGE 4D data sets.
> The Zeiss system in Live mode is capable of generating up to 120 FPS (XY)
> and/or fast Z-stacks (approx 8-10um/sec).
> We are satisfied customers and have no commercial interest in Zeiss.
> Rick,
>
> Richard Harris, Manager - Integrated Microscopy @ Biotron
> The Biotron - Center for Experimental Climate Change Research
> University of Western Ontario,
> London Ontario, CANADA.
> N6A 5B7
> Ph.  519-661-2111 ext. 86780
> Fax  [phone]
> e-mail [hidden email]
> web: www.thebiotron.ca
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]] On
> Behalf Of Zoltan Cseresnyes
> Sent: Monday, January 11, 2010 8:31 AM
> To: [hidden email]
> Subject: Live cell imaging experiences, please
>
> Dear All,
>
> We are planning to run a few tests, where we follow cell development
> in 8-9 XY positions, running 45 um Z stacks (step size 2.5 um), 2.5
> min time interval, for a total of 14 hours at room temp, using GFP and
> DsRed settings.  We had lots of success with the Leica SP5 system for
> the past 2 years.  We are wondering if this community has more input
> about how other systems would perfom here?  We are most interested in
> Olympus FV1000 and Nikon A1.  Our main concerns are the simplicity of
> the setting up procedure, the stability of the system during the long
> scans, and the handling of the large data files.
>  All inputs will be much appreciated!  Happy New Year to all!  With
> best wishes,
>
> Zoltan
>
> --
>
> Zoltan Cseresnyes
> Facility manager, Imaging Suite
> Univ. Cambridge, UK
>

--

Zoltan Cseresnyes
Facility manager, Imaging Suite