What has happened to "extended focus" in Leica Software?
Posted by Torsten.Fregin on
URL: http://confocal-microscopy-list.275.s1.nabble.com/What-has-happened-to-extended-focus-in-Leica-Software-tp4427300.html
Hey,
a "long" time ago I used an Leica TCS NT confocal, and I remember that I was able to view
the 3D stacks with two differend methods in 2D: "Extended Focus" and "Projection",
respectively.
With our new Leica Confocal with the new LAS AF software, I have just one way to view the
stacks: "Maximum projection", which gives me results same as with the old "Projection". (If I
open old .tiff files and process them)...
The old "ExtFoc" worked for me quite well sometimes, the new "Maximum Projection" very
often needs deconvolution to get a clear, that is, crisp image...
Does anyone remember what the difference was between the two modes in the old software?
:-) Torsten