Posted by
Jonathan Boyd on
URL: http://confocal-microscopy-list.275.s1.nabble.com/What-has-happened-to-extended-focus-in-Leica-Software-tp4427300p4427571.html
Hi Torsten,
Under the Processing tab go to 3D projection. Look under the dropdown tab. It defaults to Max projection. You will see there are other methods for rendering your volumes in this dropdown.
Hope this helps,
Jonathan
Jonathan Boyd
Confocal Application Specialist - Life Science Division
Leica Microsystems, Inc.
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http://www.leica-microsystems.com/lsdspecial----- Original Message -----
From: Torsten.Fregin
Sent: 01/21/2010 04:38 PM CET
To:
[hidden email]
Subject: What has happened to "extended focus" in Leica Software?
Hey,
a "long" time ago I used an Leica TCS NT confocal, and I remember that I was able to view
the 3D stacks with two differend methods in 2D: "Extended Focus" and "Projection",
respectively.
With our new Leica Confocal with the new LAS AF software, I have just one way to view the
stacks: "Maximum projection", which gives me results same as with the old "Projection". (If I
open old .tiff files and process them)...
The old "ExtFoc" worked for me quite well sometimes, the new "Maximum Projection" very
often needs deconvolution to get a clear, that is, crisp image...
Does anyone remember what the difference was between the two modes in the old software?
:-) Torsten
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