Re: What has happened to "extended focus" in Leica Software?

Posted by rjpalmer on
URL: http://confocal-microscopy-list.275.s1.nabble.com/What-has-happened-to-extended-focus-in-Leica-Software-tp4427300p4433638.html

Torsten - I meant ask Leica about the algorithm, which does not belong to the two "simple" ways to do a projection (maximum or average).

Thanks everyone for the replies!

Guy - I did the deconvolution on the whole stack, not the projection... ;-)

But I still don't know what kind of algorithm Leica used for the "Extended Focus" function in the old software package. Today I took my old .tif stacks and produced new projections (maximum and average) with the Leica LAS package, but they don't look as the old pictures I still have on my hard disk (from the old equipment)... So I get three different projection versions from a single stack.

Robert - I contacted Leica to get another licence key, as it is depends on a hardware key the setup program of the TCS NT PowerScan software produces...  (I try to install the old package on a modern PC; Win NT works fine so far). Actually, I send the first email to the list b/c I am almost always surprised about the responses... Over the years I learned a lot from the list...:-)

Glen - Thanks for this hint, I will give the ImageJ plugin a try asap!

:-)  Torsten

 

-- 

Robert J. Palmer Jr., Ph.D.
Natl Inst Dental Craniofacial Res - Natl Insts Health
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