>Guy - I did the deconvolution on the whole stack, not the
projection... ;-)
Phew, that’s a relief!
>But I still don't know what kind of algorithm Leica used
for the "Extended Focus" function in the old software package. Today
I took my old .tif stacks and produced new projections (maximum and average)
with the Leica LAS package, but they don't look as the old pictures I still
have on my hard disk (from the old equipment)... So I get three different
projection versions from a single stack.
As others have suggested, try using some other package such as
Image J where the functions are well defined and see what they do.
Essentially there are three ways of projecting a series – average
and maximum as I mentioned before, and ‘alpha-blending’ or ‘rendering’
where you assign more prominence to the voxels at the top of the stack –
ie those ‘closest’ to you. Once you get into this there are several
algorithms and endless possible variations of settings and no two packages will
give the same result. Maybe Leica are doing something like this
now?
The only other thing I can think of is that in the old NT
software Leica were doing an average with insufficient bit depth so that
there were rounding errors – it seems unlikely that they would do that
now.
Guy
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