Re: stage motorization

Posted by Cameron Nowell on
URL: http://confocal-microscopy-list.275.s1.nabble.com/stage-motorization-tp4455148p4456938.html

Hi Charles,
 
A motorised stage can also be used to create montage/tile scans of larger areas. This can give some pretty impressive resultsbut can take a long time to capture (e.g 4x4 fields of view with 30 slices is 16 normal Z series captures).
 
 
Cheers
 
 
Cam
 
 
Cameron J. Nowell
Microscpy Manager
Central Resource for Advanced Microscopy
Ludwig Insttue for Cancer Research
PO Box 2008
Royal Melbourne Hospital
Victoria, 3050
AUSTRALIA
 
Office: [phone]
Mobile: [phone]
Fax: [phone]
 
http://www.ludwig.edu.au/branch/research/platform/microscopy.htm
 

________________________________

From: Confocal Microscopy List on behalf of Charles Stevens
Sent: Tue 26/01/2010 3:56 AM
To: [hidden email]
Subject: stage motorization



Dear List,
 
   We're planning to set up an inverted confocal microscope for live cell imaging. I'd appreciate any opinions on using manual vs x-y motorized stage. Is the motorized stage more stabile during long-term experiments? Does it facilitate/is it necessary for any experiments besides multi-point time-lapse imaging?
   Thanks for your comments!
 
  Charles