Posted by
Oliver Biehlmaier on
Feb 01, 2010; 9:44am
URL: http://confocal-microscopy-list.275.s1.nabble.com/LSM-710-tp4493320p4493413.html
Dear Axel,
we are using an LSM 710 at our facility for a year now and we are very happy
with it.
The sensitivity is much higher than in the previous 510 systems. We can use
very low laser power and still obtain nice and clear images which means that
you can keep phototoxicity low at this system. Thus, we have more and more
people using the system for live cell imaging - especially for
photoactivation experiments.
Unfortunately, I can neither provide you with any quantitative results
concerning the sensitivity nor any link where you could look it up. I can
just tell you that we experience the 710 as a very sensitive system. It is
definitively more sensitive than our Zeiss LSM510s and Leica SP2s. However,
we do not have a SP5, Nikon A1 or Olympus FV1000 at our facility so I can
not compare the sensitivity of a those vs. the Zeiss710.
Cheers,
Oliver
P.S.: I have no commercial interests.
<><><><><><><><><><><><><><><><><><><><><><><><>
Oliver Biehlmaier, PhD
Advanced Microscopy
Light Microscopy Centre (LMC)
Schafmattstr. 18
ETH-Hönggerberg, HPM F16
8093 Zürich
Switzerland
Phone: +41 44 63 23020
Fax: +41 44 63 21298
Email:
[hidden email]
URL:
http://www.lmc.ethz.ch/People/Oliver<><><><><><><><><><><><><><><><><><><><><><><><>
On 01.02.10 10:05, "Axel Preuss" <
[hidden email]> wrote:
> I have found some posts about the LSM 710 in the listserv, but have not
> noticed yet an assessment by users of this system.
> While Zeiss stresses the efficiency of the beam path in the 710, and indeed
> the concept looks impressive, I wonder whether anybody knows about the PMT
> sensitivity of the system. Who manufactures the PMTs, and is there any place
> I can look up their sensitivity? A user of our facility has asked me how the
> 710 (sensitivity) would stack up in comparison to other confocals.Probably
> the answer is not easy, as S/N, beam path length, grating efficiency etc
> come into play. Still, who has had the chance to compare the 710 with
> others, like SP5 or Olympus?