Re: LSM 710

Posted by Andreas Bruckbauer on
URL: http://confocal-microscopy-list.275.s1.nabble.com/LSM-710-tp4493320p4497139.html

I tried to measure the sensitivity of the LSM 710 by spreading individual Cy3 labelled antibody fragments onto glass slides. With low scanning speeds one gets excellent images, while this was not possible on  the Olympus FV1000 spectral detecorand much better than the filter based detector on the FV1000. However this is more a measure of the suppression of laser light than sensitivity. Furthermore there is now the LSM 780 with GaAsP spectral detector which is 2-3x more sensitive tan the LSM 710 (about 45% quantum efficiency).

-----Original Message-----
From: Axel Preuss <[hidden email]>
To: [hidden email]
Sent: Mon, 1 Feb 2010 9:05
Subject: LSM 710

I have found some posts about the LSM 710 in the listserv, but have not

noticed yet an assessment by users of this system.

While Zeiss stresses the efficiency of the beam path in the 710, and indeed

the concept looks impressive, I wonder whether anybody knows about the PMT

sensitivity of the system. Who manufactures the PMTs, and is there any place

I can look up their sensitivity? A user of our facility has asked me how the

710 (sensitivity) would stack up in comparison to other confocals.Probably

the answer is not easy, as S/N, beam path length, grating efficiency etc

come into play. Still, who has had the chance to compare the 710 with

others, like SP5 or Olympus?