Posted by
Christian Schumann on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Fourier-Analysis-of-an-image-sequence-tp4523929p4524870.html
Hi Jerome,
MATLAB is the tool I'd use. Depending on your image format, you can use the LOCI Bio-Formats tools and the bfopen script to get the complete time sereis into MATLAB. After organizing the images as 3D-array, you can use MATLAB's fft function along the time axis of your array (there's an optional argument that can be passed to the fft specifying the dimension along which to compute the transform). This will be pixel-wise automtatically.
Regards,
Christian
Dr. Christian Schumann
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Von: Confocal Microscopy List im Auftrag von Jerome Carpenter
Gesendet: Sa 06.02.2010 04:52
An:
[hidden email]
Betreff: [CONFOCALMICROSCOPY] Fourier Analysis of an image sequence
Hello all,
I have an image sequence and would like to take a Fourier Transform of each pixel in time (through the sequence). The goal is to generate frequency and phase maps of a field of beating cilia. I'm currently using ImageJ and the time series analyzer to get average intensity over ROIs, and Matlab for the fourier transform, but would like to go pixel by pixel. Any advice on image analysis packages/plugins to accomplish this?
Thanks!
Jerome