Re: Olympus FV1000 vs Nikon A1

Posted by Bjorn Tyrberg on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Olympus-FV1000-vs-Nikon-A1-tp4567055p4580860.html

I had several years of experience with FV1000 when we decide to buy the A1Rsi with
VAAS detector. I agree with previous posts and in a few direct comparison tests the A1
performed better, particularly at high spatial resolution. With the VAAS, images at high
resolution are even crisper and very impressing. The only down side is the software
which still has some growing problems, although most have been fixed since we got the
system about 1.5 years ago. One other thing to keep in mind is that the resonant scanner
clearly is not always a good substitution for a spinning disc. It is indeed fast and sensitive
enough for most live cell imaging needs, but noise is very apparent without averaging,
meaning that if you want spatial resolution, speed is significantly reduced by averaging.

Bjorn
Scientific Advisor, Imaging core, Sanford-Burnham Institute, Orlando, FL

On Sun, 14 Feb 2010 16:31:55 +0100, Alberto Diaspro <[hidden email]> wrote:

>In addition to my direct comments, i do agree
>AD
>
>p.s. the tirf section is also better, from my viewpoint
>Il giorno 14/feb/2010, alle ore 16.16, Roshma Azeem ha scritto:
>
>>
>> Dear Pedro,
>>
>> I have used both the machines and satisfied with the performance of both. However,

Nikon A1's built quality and performance is far better than Olympus FV 1000. If you are
planning to buy a system with spectral detector, then I would like to say that A1si or
A1Rsi would stand apart due to 32 channel multi-anode PMT.
>>
>> The image quality and sensitivity of A1 is really cool. The continuously variable 1X to
1000X zoom of A1 gives good flexibility. I feel more comfortable with the hexagonal
pinhole of A1 that gives better images. Most importantly, the spectral detector has
multiple gratings that allows the spectral step size of 2.5nm, 6nm and 10nm.
>>
>> I think only the A1 confocal has fiber connected detectors that is separated from scan
head. I have never experienced data loss or scan head induced artifacts or thermal noise.

>>
>> Want to hear the experiences of other users of these machines.
>>
>> Roshma.
>>
>> PS: No commercial interest.  
>>
>>
>>
>>
>> On Sat, Feb 13, 2010 at 10:57 PM, Pedro J Camello <[hidden email]> wrote:
>> Hi all,
>>
>> has anybody in the list performed some real comparison between Olympus
>> FV1000 and Nikon A1? We´re in the proccess of purchase a general-use
>> confocal, and any input (either on or off-list) will be very wellcome.
>>
>> Thanks
>>
>>
>> --
>> Dr Pedro J Camello
>> Dpt Physiology
>> Faculty of Veterinary Sciences
>> University of Extremadura
>> 10071 Caceres
>> Spain
>> Ph: 927257000 Extension 51321/51290
>> Fax:[phone]
>>
>
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