PSF bead shot but X Y and Z dont match

Posted by Matiar Jafari on
URL: http://confocal-microscopy-list.275.s1.nabble.com/PSF-bead-shot-but-X-Y-and-Z-dont-match-tp4589883.html

Hey Fellow ListServers,

I purchased a PSF kit from Invitrogen and imaged the beads and when i
loaded the beads to correct the Z blur i measured the XY of the bead
to make sure the diameter matched but it turns out it doesnt.  The
beads are supposedly .17um with +-.005 but whatever angle i measure
the bead at in xy i keep getting .4 um.  Any ideas as to why this is
happening?

Thank You
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Matiar Jafari