Re: chlorophyll and associated pigment spectra

Posted by Rosemary.White on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Microscopy-used-equipment-tp4594144p4596438.html

Hi Christian,

If you use the 633 laser, you’ll get the expected emission peak at around 695 nm which is largely photosystem II emission, then a flat tail up to 760 or so which is mostly PSI.  There are many absorption and emission spectra around, though I suspect quite a few of these are for isolated pigments in a polar solvent.  The emission spectra depend very strongly on the wavelength(s) of the excitation light, so there isn’t really a standard emission spectrum.

cheers,
Rosemary

Rosemary White
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

T 61 2 6246 5475
F 61 2 6246 5334
M 61 2 420 972 028



On 19/02/10 5:33 PM, "Christian" <celowsky21@...> wrote:

Does anyone have the autofluorescent spectra for plants due to the pigments in the chloroplasts?

I know there is a very strong peak beginning in the 600 nm range, but there is also one in the 520 nm range as well when excited with 488 nm. I have not been able to locate it on the usual spectral sites. Thanks Christian