Calcium imaging in Multiphoton systems

Posted by Carlos Sanchez-8 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Calcium-imaging-in-Multiphoton-systems-tp4598586.html

Hello, good afternoon.

 

- From your experience, which are the best calcium dyes to be used in Multiphoton systems? Calcium green, perhaps?

 

- I supposed that excitation ratio dyes like Fura-2 are not recommended but has anybody used it with multiphoton excitation? Is it worthy?

 

Thank you very much in advance for your help.

Regards,

 

Carlos Sánchez Martín

Servicio de Microscopía Optica y Confocal (Lab.310)

Centro de Biología Molecular "Severo Ochoa" (CSIC-UAM)

C/Nicolás Cabrera,1

Universidad Autónoma de Madrid. Cantoblanco.

28049. Madrid

Telf: 91 196 4613/4643

Fax: [phone]

E-mail: [hidden email]

http://www.cbm.uam.es/confocal