Re: Calcium imaging in Multiphoton systems

Posted by Mark Cannell on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Calcium-imaging-in-Multiphoton-systems-tp4598586p4600007.html

Indo-1 is good.

See
Jones, K.T., Soeller, C. & Cannell, M.B. (1998) The passage of Ca2+ and
fluorescent markers between the sperm and egg after fusion in the mouse.
Development 125, 4627-4635.

(shameless self promotion I know ;-)

Cheerrs



Carlos Sanchez wrote:

>
> Hello, good afternoon.
>
>  
>
> - From your experience, which are the best calcium dyes to be used in
> Multiphoton systems? Calcium green, perhaps?
>
>  
>
> - I supposed that excitation ratio dyes like Fura-2 are not
> recommended but has anybody used it with multiphoton excitation? Is it
> worthy?
>
>  
>
> Thank you very much in advance for your help.
>
> Regards,
>
>  
>
> Carlos Sánchez Martín
>
> Servicio de Microscopía Optica y Confocal (Lab.310)
>
> Centro de Biología Molecular "Severo Ochoa" (CSIC-UAM)
>
> C/Nicolás Cabrera,1
>
> Universidad Autónoma de Madrid. Cantoblanco.
>
> 28049. Madrid
>
> Telf: 91 196 4613/4643
>
> Fax: [phone]
>
> E-mail: [hidden email]
>
> http://www.cbm.uam.es/confocal
>
>  
>