Dr. Martin,
I can send a recent 4 page brief (or link
to the online version) on 2-photon compatible dyes for neuronal physiology. (It
was the cover story in our BioProbes 60 issue last fall.)
Oregon Green Bapta 1 and 2 and the fluo
series are popular for Multi-bolus labeling as they are cell permeant and
non-ratiometric. For intracellular injection there are many choices, depends
on what calcium range you are monitoring, whether you are seeking absolute
concentrations or just monitoring deltas. This and more is covered in our
review.
Kind Regards,
Mike Ignatius,
Molecular Probes/Lifetech
From: Confocal
Microscopy List [mailto:[hidden email]] On Behalf Of Carlos Sanchez
Sent: Friday, February 19, 2010
7:49 AM
To:
[hidden email]
Subject: Calcium imaging in
Multiphoton systems
Hello, good afternoon.
- From your experience, which are the best calcium
dyes to be used in Multiphoton systems? Calcium green, perhaps?
- I supposed that excitation ratio dyes like Fura-2
are not recommended but has anybody used it with multiphoton excitation? Is it
worthy?
Thank
you very much in advance for your help.
Regards,
Carlos Sánchez Martín
Servicio de Microscopía Optica y Confocal (Lab.310)
Centro de Biología Molecular "Severo Ochoa" (CSIC-UAM)
C/Nicolás Cabrera,1
Universidad Autónoma de Madrid. Cantoblanco.
28049. Madrid
Telf:
91 196
4613/4643
Fax: 91 196 4420
E-mail: [hidden email]
http://www.cbm.uam.es/confocal
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