Posted by
aseem mishra on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Fluoview-1000-and-FCS-tp4594644p4603452.html
Hi Jean,
You might want to have a look at Enrico Gratton's work and specially
SimFCS wherein one can calculate diffusion coefficients from an image
collected in a point-scan mode. I do not know if a blinking laser
gives you the possibility of doing FCS (Do let me know if such a
possibility exists. I would love to try it at my place.) We rather use
a hardware correlator and a simple laser (diode/HeNe)
Aseem
On Fri, Feb 19, 2010 at 3:27 AM, Jean-Pierre CLAMME
<
[hidden email]> wrote:
>
> Hi all,
>
> I'm thinking about modifying our fluoview 1000 to do FCS. The fluoview has
> the possibility to do single point measurement. However as we can still
> choose the pixel time in this mode, I was wondering if the laser beam always
> stays at the same point of interest and continuously illuminates the point
> or if it actually "blinks".
>
> If it doesn't "blink", I would think it could hinder the FCS measurement et
> thus I'm wondering if there is an option to change this in the soft. ?
>
> Thanks,
>
> JP
>
>
>
>
> - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
> Jean-Pierre CLAMME, PhD
> Senior Scientist
> Nitto Denko Technical
> 501 Via Del Monte
> Oceanside, CA 92058
> E-mail:
[hidden email]
> Phone: +760.435.7065
>
--
Aseem Mishra
Senior Research Assistant,
Malaria Group,
International Centre for Genetic Engineering and Biotechnology,
New Delhi-110067
INDIA