Re: Calcium imaging in Multiphoton systems
Posted by
Roland Nitschke on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Calcium-imaging-in-Multiphoton-systems-tp4598586p4612067.html
Hello,
we have used fura-2 with single wavelength 2-P excitation here:
Simultaneous Measurements of Cytosolic and Mitochondrial Ca2+
Transients in HT29 Cells*
Sigrid Ricken, Jens Leipziger, Rainer Greger, and Roland Nitschke‡
THE JOURNAL OF BIOLOGICAL CHEMISTRY Vol. 273, No. 52, Issue of December
25, pp. 34961–34969, 1998
If you have access to a double 2-P system also excitation ratioing
should be possible using app. 690 and 780 nm.
Regards
Roland
Am 19.02.2010 16:49, schrieb Carlos Sanchez:
Hello, good afternoon.
-
>From your
experience, which are the best calcium dyes to be used in Multiphoton
systems? Calcium
green, perhaps?
-
I supposed
that excitation ratio dyes like Fura-2 are not recommended but has
anybody used
it with multiphoton excitation? Is it worthy?
Thank you very much in
advance for your
help.
Regards,
Carlos
Sánchez
Martín
Servicio de
Microscopía
Optica y Confocal (Lab.310)
Centro de
Biología Molecular
"Severo Ochoa" (CSIC-UAM)
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Universidad
Autónoma de
Madrid. Cantoblanco.
28049. Madrid
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Life Imaging Center
Centre of Systems Biology
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