On Fri, Feb 26, 2010 at 10:32 PM, Tim Feinstein <
[hidden email]> wrote:
> It is worth noting that a two photon module now exists for the A1 resonance
> head. This might make for a very fast intravital/tissue slice imaging rig.
> I would be interested to know whether anyone has demoed it yet.
> cheers,
>
> Tim
>
>
> On Feb 26, 2010, at 11:45 PM, Roshma Azeem wrote:
>
> A1 with spectral detector can do most of the job for live cell imaging
> related applications.
>
> Noise is unavoidable when we go for high speed. Moreover, resonant scanner
> is inherently prone to make noise. Noise correction averaging has to be
> tuned to get better signals. This would come in due course by practicing.
>
> A1R is a different machine from A1 in terms of the scan head. There is no
> possibility to upgrade the A1 into A1R in future. The decision need to be
> taken at the time of purchase. This is a limitation for those who are
> looking for future upgradation.
>
> Roshma.
>
>
>
>
> On Fri, Feb 26, 2010 at 3:45 AM, Vergara, Leoncio A. <
[hidden email]>
> wrote:
>>
>> Depends on what you want to do.
>>
>> Obviously the resonant scanned allows you to capture images at higher
>> speed, this does not mean only the ability to follow up faster events, but
>> also you can capture faster z-stacks and multi-location experiments.
>>
>> The resonant scanner can also be used for fast FRAP experiments, you can
>> image with the resonant scanner while using the conventional scanner to
>> photobleachwithout having to interrupt image capture. I only ried it once
>> and it seems to work well. The limitation os that it only works with 405nm
>> as I understand.
>>
>> Other than that, you need to have in moind the limitations: faster
>> scanning means worst signal to noise ratio and you don't have all the
>> versatility of conventional scanners...
>>
>> ...unfortunately I understand that you have to make the decision at the
>> moment of purchase, I don't think you can add it later... (?)
>>
>> -----Original Message-----
>> From: Confocal Microscopy List [mailto:
[hidden email]]
>> On Behalf Of Amol Karwa
>> Sent: Thursday, February 25, 2010 3:43 PM
>> To:
[hidden email]
>> Subject: Re: Olympus FV1000 vs Nikon A1
>>
>> Hello everyone,
>>
>> Well right now I don't have anything substantial to add to the discussion
>> except that we were in similar situation and we are going with Nikon A1. The
>> decision was simply based on our imaging needs and the superiority of Nikon
>> A1. The instrument is being installed as we speak and I'm really excited to
>> use it. It will be pretty big jump from Leica SP2 to this A1
>>
>> However I do have a question and this comes from the inexperience. What
>> advantage that A1r scanhead provides vis a vis A1? I know it has high speed
>> imaging capabilities but would you say A1 can do most of the job for live
>> cell imaging?
>>
>> Thanks,
>> Amol
>> --
>> View this message in context:
>>
http://n2.nabble.com/Olympus-FV1000-vs-Nikon-A1-tp4567055p4635601.html
>> Sent from the Confocal Microscopy List mailing list archive at Nabble.com.
>
>
>