Re: Zeiss or Olympus

Posted by Mark Cannell on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Zeiss-or-Olympus-tp4702418p4705967.html

Hi RICHARD

As far as I know, for PMT based systems the dynamic range does not
depend on PMT sensitivity (which is determined by the photocathode) but
on gain (=PMT HV) and A/D settings.

Cheers Mark

BURRY wrote:

>
> Vladimir and Stephan
>
>  
>
> The Zeiss 780 is an upgrade for the single photon.  The big issues for
> me with the Zeiss and Olympus multiphoton are the detector dynamic
> range, and detector sensitivity.  Extremely sensitive detectors can
> have a limited dynamic range.  I would be interested in hearing from
> someone who had recently tested not only the sensitivity of these two
> multiphotons, but also the dynamic range in an image collected a
> moderate to low fluorescence levels.  My evaluation a year ago was
> that the Olympus multiphoton had the better combination of sensitivity
> and dynamic range.
>
>  
>
> Dick Burry
>
>
> ----- Original Message -----
> From: Stephan Speier <[hidden email]>
> Date: Tuesday, March 9, 2010 8:10 am
> Subject: Re: Zeiss or Olympus
> To: [hidden email]
>
> > Dear Vladimir,
> >
> > We tested the Zeiss LSM710 and the Olympus FV1000 regarding
> > confocal
> > and multiphoton imaging and had already decided for the FV1000
> > when
> > Zeiss released the LSM780.
> > After testing the LSM780 we changed our mind and decided for Zeiss.
> > For our purposes the LSM780 has exactly the improvements over
> > the 710,
> > we were looking for and therefore beat the Olympus.
> >
> > Best,
> > Stephan
> >
> >
> > ----- Original Message -----
> > From: Vladimir Gukassyan <[hidden email]>
> > Date: Tuesday, March 9, 2010 1:18 pm
> > Subject: Zeiss or Olympus
> > To: [hidden email]
> >
> > > Dear List Members,
> > >
> > > We're making a selection between Zeiss 710 and Olympus FV1000
> > for the
> > > multiphoton imaging.
> > > I would be thankful for all user experience cases and
> > suggestions on
> > > this matter.
> > >
> > > Thank you in advance.
> > > With regards,
> > > Vladimir
> > >
> > > ---------------------------------
> > > Vladimir Ghukasyan
> > > Confocal and Multiphoton Imaging Core,
> > > Neuroscience Research Center
> > > University of North Carolina
> > > 115 Mason Farm Rd., Bld. 245, 7 Fl.
> > > Chapel Hill, NC 27599-7250
> > > Tel.: [phone]
> > >
> >
> >
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