http://confocal-microscopy-list.275.s1.nabble.com/Zeiss-or-Olympus-tp4702418p4706176.html
I was thinking about the use of GaAs ( gallium asrenide) detectors for multiphoton by Zeiss for the NLO. This are not a PMT and have very different properties.
> Hi RICHARD
>
> As far as I know, for PMT based systems the dynamic range does
> not
> depend on PMT sensitivity (which is determined by the
> photocathode) but
> on gain (=PMT HV) and A/D settings.
>
> Cheers Mark
>
> BURRY wrote:
> >
> > Vladimir and Stephan
> >
> >
> >
> > The Zeiss 780 is an upgrade for the single photon. The
> big issues for
> > me with the Zeiss and Olympus multiphoton are the detector
> dynamic
> > range, and detector sensitivity. Extremely sensitive
> detectors can
> > have a limited dynamic range. I would be interested in
> hearing from
> > someone who had recently tested not only the sensitivity of
> these two
> > multiphotons, but also the dynamic range in an image collected
> a
> > moderate to low fluorescence levels. My evaluation a
> year ago was
> > that the Olympus multiphoton had the better combination of
> sensitivity
> > and dynamic range.
> >
> >
> >
> > Dick Burry
> >
> >
> > ----- Original Message -----
> > From: Stephan Speier <
[hidden email]>
> > Date: Tuesday, March 9, 2010 8:10 am
> > Subject: Re: Zeiss or Olympus
> > To:
[hidden email]> >
> > > Dear Vladimir,
> > >
> > > We tested the Zeiss LSM710 and the Olympus FV1000 regarding
> > > confocal
> > > and multiphoton imaging and had already decided for the FV1000
> > > when
> > > Zeiss released the LSM780.
> > > After testing the LSM780 we changed our mind and decided for
> Zeiss.> > For our purposes the LSM780 has exactly the
> improvements over
> > > the 710,
> > > we were looking for and therefore beat the Olympus.
> > >
> > > Best,
> > > Stephan
> > >
> > >
> > > ----- Original Message -----
> > > From: Vladimir Gukassyan <
[hidden email]>
> > > Date: Tuesday, March 9, 2010 1:18 pm
> > > Subject: Zeiss or Olympus
> > > To:
[hidden email]> > >
> > > > Dear List Members,
> > > >
> > > > We're making a selection between Zeiss 710 and Olympus FV1000
> > > for the
> > > > multiphoton imaging.
> > > > I would be thankful for all user experience cases and
> > > suggestions on
> > > > this matter.
> > > >
> > > > Thank you in advance.
> > > > With regards,
> > > > Vladimir
> > > >
> > > > ---------------------------------
> > > > Vladimir Ghukasyan
> > > > Confocal and Multiphoton Imaging Core,
> > > > Neuroscience Research Center
> > > > University of North Carolina
> > > > 115 Mason Farm Rd., Bld. 245, 7 Fl.
> > > > Chapel Hill, NC 27599-7250
> > > > Tel.: +1 919 966 5807
> > > >
> > >
> > >
> > > --
> > >
>
>
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Richard W. Burry, Ph.D.