Just to mention, should one be stuck with PMTs instead GaAs, one could play with the applied bias voltage to modify
dark noise (to
balance the gain versus noise. )
Nice thing with Olympus Kalman filtering is that its use would allow increase the bias voltage of PMT
Thanks
Axel
Central Imaging (IMCB) 6-19B, cell
+65 9271.5622
From: Confocal Microscopy List [mailto:[hidden email]]
On Behalf Of RICHARD BURRY
Sent: Wednesday, March 10, 2010 8:17 AM
To: [hidden email]
Subject: Re: Zeiss or
Mark
I was thinking about the use of GaAs ( gallium asrenide) detectors for multiphoton by Zeiss for the NLO. This are not a PMT and have very different properties.
Dick Burry
----- Original Message -----
From: Mark Cannell <[hidden email]>
Date: Tuesday, March 9, 2010 6:33 pm
Subject: Re: Zeiss or
To: [hidden email]
> Hi RICHARD
>
> As far as I know, for PMT based systems the dynamic range does
> not
> depend on PMT sensitivity (which is determined by the
> photocathode) but
> on gain (=PMT HV) and A/D settings.
>
> Cheers Mark
>
> BURRY wrote:
> >
> > Vladimir and Stephan
> >
> >
> >
> > The Zeiss 780 is an upgrade for the single photon. The
> big issues for
> > me with the Zeiss and
> dynamic
> > range, and detector sensitivity. Extremely sensitive
> detectors can
> > have a limited dynamic range. I would be interested in
> hearing from
> > someone who had recently tested not only the sensitivity of
> these two
> > multiphotons, but also the dynamic range in an image collected
> a
> > moderate to low fluorescence levels. My evaluation a
> year ago was
> > that the
> sensitivity
> > and dynamic range.
> >
> >
> >
> > Dick Burry
> >
> >
> > ----- Original Message -----
> > From: Stephan Speier <[hidden email]>
> > Date: Tuesday, March 9, 2010 8:10 am
> > Subject: Re: Zeiss or
> > To: [hidden email]
> >
> > > Dear Vladimir,
> > >
> > > We tested the Zeiss LSM710 and the Olympus FV1000 regarding
> > > confocal
> > > and multiphoton imaging and had already decided for the FV1000
> > > when
> > > Zeiss released the LSM780.
> > > After testing the LSM780 we changed our mind and decided for
> Zeiss.> > For our purposes the LSM780 has exactly the
> improvements over
> > > the 710,
> > > we were looking for and therefore beat the
> > >
> > > Best,
> > > Stephan
> > >
> > >
> > > ----- Original Message -----
> > > From: Vladimir Gukassyan <[hidden email]>
> > > Date: Tuesday, March 9, 2010 1:18 pm
> > > Subject: Zeiss or
> > > To: [hidden email]
> > >
> > > > Dear List Members,
> > > >
> > > > We're making a selection between Zeiss 710 and Olympus FV1000
> > > for the
> > > > multiphoton imaging.
> > > > I would be thankful for all user experience cases and
> > > suggestions on
> > > > this matter.
> > > >
> > > > Thank you in advance.
> > > > With regards,
> > > >
> > > >
> > > > ---------------------------------
> > > > Vladimir Ghukasyan
> > > > Confocal and Multiphoton Imaging Core,
> > > >
> > > >
> > > >
> > > >
> > > > Tel.: +1 919 966 5807
> > > >
> > >
> > >
> > > --
> > >
>
>
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Richard W. Burry, Ph.D.
Department of Neuroscience, College of Medicine
Campus Microscopy and Imaging Facility, Director
The Ohio State University
Associate Editor, Journal of Histochemistry and Cytochemistry
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