Re: FRET - PMT Spectral Sensitivity

Posted by leoncio vergara on
URL: http://confocal-microscopy-list.275.s1.nabble.com/FRET-PMT-Spectral-Sensitivity-tp4720210p4720296.html

... the quantum efficiency of the detector versus wavelenght is something you would need to get from the manufacturer... unfortunately this may be a proprietary piece of information in most cases...

you need this information to calculate the FRET efficiency...

________________________________________
From: Confocal Microscopy List [[hidden email]] On Behalf Of Pablo German [[hidden email]]
Sent: Thursday, March 11, 2010 9:17 PM
To: [hidden email]
Subject: FRET - PMT Spectral Sensitivity

Hi,

I'm analysing FRET images using the pFRET plugin of ImageJ developed
at Universit of Virginia.

When setting the parameters it asks me for the PMT Spectral
Sensitivity for donor and acceptor. I'm using eCFP and eYFP. Where can
I find these values? Is it important?

Regards,
Pablo

--
Pablo German
PhD Candidate

Plant and Food Research
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