Re: TIRF objective for routine imaging

Posted by John Oreopoulos on Mar 22, 2010; 2:47pm
URL: http://confocal-microscopy-list.275.s1.nabble.com/TIRF-objective-for-routine-imaging-tp4778151p4778221.html

There's no reason why you can't do this, and the images will be fine,  
but if you're looking to do 3D sectional imaging of the RBCs, you  
might be better off with a high NA water immersion objective instead  
which will have lower spherical aberrations - you likely will get  
better signal with that type objective. Try comparing both types of  
objectives if possible.

John Oreopoulos


On 22-Mar-10, at 5:29 AM, Charu Tanwar wrote:

> Dear List
>
> Anybody please let me know whether we can use TIRF 100XH (N.A.
> 1.49,Working Distance 0.12mm,Coverglass correction 0.13-.20mm -  
> this is a
> special TIRF objective) for routine confocal imaging of bacteria  
> and RBC's with
> low flourescence signal.
> Can it be mounted on a confocal microscope from TIRF microscope if  
> both the
> systems are from same company.
>
> Thanks in advance
>
> Charu Tanwar
> Imaging Specialist
> Advanced Instrumentation Research Facility
> Jawaharlal Nehru University
> New Delhi
> India.