Re: TIRF objective for routine imaging

Posted by Marco Dal Maschio-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/TIRF-objective-for-routine-imaging-tp4778151p4780054.html

Dear Charu,

I had the opportunity to test from the same company 60x 1.45tirf // 60x VC // 60x 1.49 tirf.

I remember that probably 60x 1.49 is not plan, related to the  flat field correction. 

But this objective is currently used by colleagues  performing tracking and imaging in neuronal cultures

with optimal image quality. If you want I can send you images comparing 60x VC and 60x 1.49 tirf.

Sorry but no experience about 100x.

best 

Marco   

On Mon, Mar 22, 2010 at 10:29 AM, Charu Tanwar <[hidden email]> wrote:

Dear List

Anybody please let me know whether we can use TIRF 100XH (N.A.
1.49,Working Distance 0.12mm,Coverglass correction 0.13-.20mm - this is a
special TIRF objective) for routine confocal imaging of bacteria and RBC's with
low flourescence signal.
Can it be mounted on a confocal microscope from TIRF microscope if both the
systems are from same company.

Thanks in advance

Charu Tanwar
Imaging Specialist
Advanced Instrumentation Research Facility
Jawaharlal Nehru University
New Delhi
India.