Hi Charu,
We have A Nikon TIRF module on our Nikon TiE so we have the Apo 60X
1.49 NA TIRF Objective, I have used this for regular confocal imaging with
great success.
Best,
Neeraj.
Neeraj V.
Gohad, Ph.D.
Postdoctoral
Fellow
Okeanos
Research Group
Department of
Biological Sciences
132 Long Hall
Clemson
University
Clemson,SC-29634
Phone:
864-656-3597
Fax:
864-656-0435
Website:
http://www.clemson.edu/okeanos
Please note
my new email address: [hidden email]
From: Confocal Microscopy
List [mailto:[hidden email]] On Behalf Of Marco Dal
Maschio
Sent: Monday, March 22, 2010 3:56 PM
To: [hidden email]
Subject: Re: TIRF objective for routine imaging
Dear Charu,
I had the opportunity to test from the same company 60x
1.45tirf // 60x VC // 60x 1.49 tirf.
I remember that probably 60x 1.49 is not plan, related to
the flat field correction.
But this objective is currently used by colleagues
performing tracking and imaging in neuronal cultures
with optimal image quality. If you want I can send you
images comparing 60x VC and 60x 1.49 tirf.
Sorry but no experience about 100x.
best
Marco
On Mon, Mar 22, 2010 at 10:29 AM, Charu Tanwar <[hidden email]> wrote:
Dear List
Anybody please let me know whether we can use TIRF 100XH (N.A.
1.49,Working Distance 0.12mm,Coverglass correction 0.13-.20mm - this is a
special TIRF objective) for routine confocal imaging of bacteria and RBC's with
low flourescence signal.
Can it be mounted on a confocal microscope from TIRF microscope if both the
systems are from same company.
Thanks in advance
Charu Tanwar
Imaging Specialist
Advanced Instrumentation Research Facility
Jawaharlal Nehru University
New Delhi
India.
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