Confocal Microscopy List - Re: TIRF objective for routine imaging

Re: TIRF objective for routine imaging

Posted by Charu Tanwar on
URL: http://confocal-microscopy-list.275.s1.nabble.com/TIRF-objective-for-routine-imaging-tp4778151p4782273.html

Thank you all for your valuable inputs.

Charu

CHARU TANWAR
Imaging Specialist
Advanced Instrumentation Research Facility
Jawaharlal Nehru University
New Delhi 110067
India.

--- On Tue, 23/3/10, Tim Feinstein <[hidden email]> wrote:

From: Tim Feinstein <[hidden email]>
Subject: Re: TIRF objective for routine imaging
To: [hidden email]
Date: Tuesday, 23 March, 2010, 5:55 AM

We use the same objective as Neeraj on a A1/TiE for confocal, widefield and TIRF. No complaints.

cheers,

Tim Feinstein

On Mar 22, 2010, at 7:39 PM, Neeraj Gohad wrote:

Hi Charu,

We have A Nikon TIRF module on our Nikon TiE so we have the Apo 60X 1.49 NA TIRF Objective, I have used this for regular confocal imaging with great success.

Best,

Neeraj.

Neeraj V. Gohad, Ph.D.

Postdoctoral Fellow

Okeanos Research Group

Department of Biological Sciences

132 Long Hall

Clemson University

Clemson,SC-29634

Phone: 864-656-3597

Fax: 864-656-0435

Website: http://www.clemson.edu/okeanos

Please note my new email address: http://in.mc83.mail.yahoo.com/mc/compose?to=neerajg@...

From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Marco Dal Maschio
Sent: Monday, March 22, 2010 3:56 PM
To: http://in.mc83.mail.yahoo.com/mc/compose?to=CONFOCALMICROSCOPY@...
Subject: Re: TIRF objective for routine imaging

Dear Charu,

I had the opportunity to test from the same company 60x 1.45tirf // 60x VC // 60x 1.49 tirf.

I remember that probably 60x 1.49 is not plan, related to the flat field correction.

But this objective is currently used by colleagues performing tracking and imaging in neuronal cultures

with optimal image quality. If you want I can send you images comparing 60x VC and 60x 1.49 tirf.

Sorry but no experience about 100x.

best

Marco

On Mon, Mar 22, 2010 at 10:29 AM, Charu Tanwar <http://in.mc83.mail.yahoo.com/mc/compose?to=tanwar_charu@...> wrote:

Dear List

Anybody please let me know whether we can use TIRF 100XH (N.A.
1.49,Working Distance 0.12mm,Coverglass correction 0.13-.20mm - this is a
special TIRF objective) for routine confocal imaging of bacteria and RBC's with
low flourescence signal.
Can it be mounted on a confocal microscope from TIRF microscope if both the
systems are from same company.

Thanks in advance

Charu Tanwar
Imaging Specialist
Advanced Instrumentation Research Facility
Jawaharlal Nehru University
New Delhi
India.

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