Re: TIRF objective for routine imaging

Posted by Barbara Foster on
URL: http://confocal-microscopy-list.275.s1.nabble.com/TIRF-objective-for-routine-imaging-tp4778151p4794408.html

Hi, all

Just remember that the 1.49 NA only works with special, high refractive index liquids. 

Barbara Foster, President and Sr. Consultant

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At 05:39 PM 3/22/2010, you wrote:

Hi Charu,
 
We have A Nikon TIRF module on our Nikon TiE so we have the Apo 60X 1.49 NA TIRF Objective, I have used this for regular confocal imaging with great success.
 
Best,
 
Neeraj.
 
Neeraj V. Gohad, Ph.D.
Postdoctoral Fellow
Okeanos Research Group
Department of Biological Sciences
132 Long Hall
Clemson University
Clemson,SC-29634
Phone: [phone]
Fax: [phone]
 
Website: http://www.clemson.edu/okeanos
 
Please note my new email address: [hidden email]
 
 
From: Confocal Microscopy List [[hidden email]] On Behalf Of Marco Dal Maschio
Sent: Monday, March 22, 2010 3:56 PM
To: [hidden email]
Subject: Re: TIRF objective for routine imaging
 
Dear Charu,
I had the opportunity to test from the same company 60x 1.45tirf // 60x VC // 60x 1.49 tirf.
I remember that probably 60x 1.49 is not plan, related to the  flat field correction.
But this objective is currently used by colleagues  performing tracking and imaging in neuronal cultures
with optimal image quality. If you want I can send you images comparing 60x VC and 60x 1.49 tirf.
Sorry but no experience about 100x.
 
best
Marco  
 
On Mon, Mar 22, 2010 at 10:29 AM, Charu Tanwar <[hidden email] > wrote:
Dear List

Anybody please let me know whether we can use TIRF 100XH (N.A.
1.49,Working Distance 0.12mm,Coverglass correction 0.13-.20mm - this is a
special TIRF objective) for routine confocal imaging of bacteria and RBC's with
low flourescence signal.
Can it be mounted on a confocal microscope from TIRF microscope if both the
systems are from same company.

Thanks in advance

Charu Tanwar
Imaging Specialist
Advanced Instrumentation Research Facility
Jawaharlal Nehru University
New Delhi
India.