Re: TIRF objective for routine imaging

Posted by Cameron, Lisa on
URL: http://confocal-microscopy-list.275.s1.nabble.com/TIRF-objective-for-routine-imaging-tp4778151p4794602.html

Yes, I concur with Kurt -

Nikon 60x and 100x 1.49 NA TIRF objectives work well with the standard 1.515
Nikon oil.


---------------------------------------
Lisa Cameron, Ph.D.
Director of Confocal and Light Microscopy
Dana Farber Cancer Institute

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On
Behalf Of Kurt Thorn
Sent: Wednesday, March 24, 2010 7:28 PM
To: [hidden email]
Subject: Re: [CONFOCALMICROSCOPY] TIRF objective for routine imaging

This isn't true - our Nikon 1.49 TIRF lens works with the standard
immersion oil.

Barbara Foster wrote:

> Hi, all
>
> Just remember that the 1.49 NA only works with special, high
> refractive index liquids.
>
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>
> Microscopy/Microscopy Education
> *7101 Royal Glen Trail, Suite A
> McKinney TX 75070
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