Re: TIRF objective for routine imaging

Posted by Tim Feinstein-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/TIRF-objective-for-routine-imaging-tp4778151p4794644.html

Acknowledging in advance that I am hardly a deconvolution expert, our Nikon 60x 1.49 provides a sharper PSF than our 40x plan fluor (hardly an apples-to-apples comparison, I know).  We measured the PSF because our blind deconvolution software (Huygens) complained about the NA/medium mismatch.  It still appeared to work fairly well.  

cheers,


Tim

Timothy Feinstein, PhD
Postdoctoral Associate
University of Pittsburgh Dept. of Pharmacology
Pittsburgh, PA

 
On Mar 24, 2010, at 7:33 PM, Cameron, Lisa wrote:

> Yes, I concur with Kurt -
>
> Nikon 60x and 100x 1.49 NA TIRF objectives work well with the standard 1.515
> Nikon oil.
>
>
> ---------------------------------------
> Lisa Cameron, Ph.D.
> Director of Confocal and Light Microscopy
> Dana Farber Cancer Institute
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]] On
> Behalf Of Kurt Thorn
> Sent: Wednesday, March 24, 2010 7:28 PM
> To: [hidden email]
> Subject: Re: [CONFOCALMICROSCOPY] TIRF objective for routine imaging
>
> This isn't true - our Nikon 1.49 TIRF lens works with the standard
> immersion oil.
>
> Barbara Foster wrote:
>> Hi, all
>>
>> Just remember that the 1.49 NA only works with special, high
>> refractive index liquids.
>>
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>>
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>
>
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