Re: TIRF objective for routine imaging

Posted by John Oreopoulos on
URL: http://confocal-microscopy-list.275.s1.nabble.com/TIRF-objective-for-routine-imaging-tp4778151p4794677.html

I think maybe Barbara was referring to the Olympus special 1.65 NA  
TIRF objective which does require special (toxic and reactive)  
immersion oil and quartz substrates. But the objective in question  
was a Nikon one which I'm not familiar with.

John Oreopoulos

On 24-Mar-10, at 7:33 PM, Cameron, Lisa wrote:

> Yes, I concur with Kurt -
>
> Nikon 60x and 100x 1.49 NA TIRF objectives work well with the  
> standard 1.515
> Nikon oil.
>
>
> ---------------------------------------
> Lisa Cameron, Ph.D.
> Director of Confocal and Light Microscopy
> Dana Farber Cancer Institute
>
> -----Original Message-----
> From: Confocal Microscopy List  
> [mailto:[hidden email]] On
> Behalf Of Kurt Thorn
> Sent: Wednesday, March 24, 2010 7:28 PM
> To: [hidden email]
> Subject: Re: [CONFOCALMICROSCOPY] TIRF objective for routine imaging
>
> This isn't true - our Nikon 1.49 TIRF lens works with the standard
> immersion oil.
>
> Barbara Foster wrote:
>> Hi, all
>>
>> Just remember that the 1.49 NA only works with special, high
>> refractive index liquids.
>>
>> *Barbara Foster, President and Sr. Consultant
>>
>> Microscopy/Microscopy Education
>> *7101 Royal Glen Trail, Suite A
>> McKinney TX 75070
>> *P: *[phone]  *Skype: *fostermme
>> *W: *www.MicroscopyEducation.com
>>
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>> And
>> don't forget:  MME is now scheduling customized, on-site courses for
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>>
>> *
>
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