Re: TIRF objective for routine imaging

Posted by Stephen Cody-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/TIRF-objective-for-routine-imaging-tp4778151p4795796.html

John is on the right track,

The Nikon TIRF Objectives including the 60 and 100X 1.49 NA, TIRF
objective use conventional oil and coverslips. Several years ago I
purchase a 60X 1.45 NA objective from Nikon to use on our C1 confocal,
for high resolution applications. It worked very but I did notice it
was not as flat as our 1.4 Plan Apo oil form the same company. Horses
for courses. BTW later we bought their White light Arc TIRF system to
go with the lens!

Cheers
Steve

On 25 March 2010 10:52, John Oreopoulos <[hidden email]> wrote:

> I think maybe Barbara was referring to the Olympus special 1.65 NA TIRF
> objective which does require special (toxic and reactive) immersion oil and
> quartz substrates. But the objective in question was a Nikon one which I'm
> not familiar with.
>
> John Oreopoulos
>
> On 24-Mar-10, at 7:33 PM, Cameron, Lisa wrote:
>
>> Yes, I concur with Kurt -
>>
>> Nikon 60x and 100x 1.49 NA TIRF objectives work well with the standard
>> 1.515
>> Nikon oil.
>>
>>
>> ---------------------------------------
>> Lisa Cameron, Ph.D.
>> Director of Confocal and Light Microscopy
>> Dana Farber Cancer Institute
>>
>> -----Original Message-----
>> From: Confocal Microscopy List [mailto:[hidden email]]
>> On
>> Behalf Of Kurt Thorn
>> Sent: Wednesday, March 24, 2010 7:28 PM
>> To: [hidden email]
>> Subject: Re: [CONFOCALMICROSCOPY] TIRF objective for routine imaging
>>
>> This isn't true - our Nikon 1.49 TIRF lens works with the standard
>> immersion oil.
>>
>> Barbara Foster wrote:
>>>
>>> Hi, all
>>>
>>> Just remember that the 1.49 NA only works with special, high
>>> refractive index liquids.
>>>
>>> *Barbara Foster, President and Sr. Consultant
>>>
>>> Microscopy/Microscopy Education
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>>> 2010.  Call me for a free assessment and quote.
>>>
>>> *
>>
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--
Stephen H. Cody