Re: deconvolution software

Posted by Vincent Schoonderwoert on
URL: http://confocal-microscopy-list.275.s1.nabble.com/deconvolution-software-tp4822758p4829908.html

*commercial interest*

Hello,

Thank you Stan for your nice overview.
I would like to add the following to what you wrote about our Huygens software.

I believe Huygens allows full scripting, so processing large number of stacks without
intervention should be possible. 

Both the Huygens Essential and Professional have a batch processor to schedule and automate large numbers of deconvolution tasks .

The Huygens Professional, like the Scripting (a package that was not mentioned), has indeed full Tcl-based scripting possibilities for processing large file series.

Then, we have also a web-based version which is the Huygens Core. The Core can work with the Huygens Remote Manager (HRM; open source) as the web-interface to schedule deconvolution tasks.

On the other hand, there is no blind deconvolution algorithm, you use either a theoretical or experimental point
spread function. Also, I do not remember if you can estimate spherical
aberration from the dataset itself, or if it is only calculated from your
input parameters (RI of the mounting medium, RI of the immersion fluid,
depth of imaging).
  

Like the theoretical PSF, the spherical aberration (SA) is not estimated from the image but based on the known microscopical and sample parameters.

We have recently added the possibility to set the distance of the first image plane to the coverslip, since the SA progressively worsens when imaging further from the coverslip.

AutoDeblur and Huygens are modular, so the cost will depend on exactly what
options you get. I would expect to pay between $6k and 10k for a
confocal-only package that does not include the scripting or surface
rendering options.
  

The Huygens Essential (32bit/64bit) with a confocal deconvolution option and the batch processor is available below this range.

Best regards,

Vincent Schoonderwoert

Hello all,

Our lab is considering purchasing some deconvolution software for cleaning
up confocal image stacks. I'm soliciting any recommendations, pro or con,
for particular software packages, based on quality of results, ease of use,
cost etc. If you prefer to send your comments directly rather than post
them, please reach me at the email below.

Thanks very much for your consideration!

Cheers,

David Stuss

[hidden email]