Posted by
Alison J. North on
URL: http://confocal-microscopy-list.275.s1.nabble.com/LaVision-TriM-multiphoton-microscope-tp4962077p4962850.html
The TriM scope is a great system. We demo-ed it here in New York a
couple of years ago. I was particularly impressed by the fact that they
shipped it out here from Germany and then had it up and running well on
live samples within about 2 hours. You can use it either in single-beam
mode if you need to image deep, or in the 64-beam mode if you need to go
fast but don't have such a requirement for depth. In the 64-beam mode I
seem to remember we were limited to about 100-150 microns in depth,
while in the regular mode we could go to around 300 microns or deeper,
depending on the tissue. But the 64-beam mode certainly is fast, so
although there are several excellent multiphoton options out in the
field now - Olympus, Prairie, Zeiss etc. - I do think that the TriM has
its own niche.
A good thing about the company is that they are extremely knowledgeable
and also very flexible about the system design - for example, they have
integrated OPO lasers on their systems for some time now. The only
downside as far as we were concerned is that it is a multiphoton-only
system, and although I can see many advantages to that myself, some of
my users were adamant about wanting visible lasers on our system as
well. If you contact Sid Ragona, their US rep,
(www.RagonaScientific.com) he can give you names of people in the US who
are using the system.
Best,
Alison
Phillips, Thomas E. wrote:
>
> I have been reading about the TriM multiphoton microscope from
> LaVision BioTec
> (
http://www.lavisionbiotec.com/en/microscopy-products/trim-scope/).
> They use a combination of mirrors and a beam splitting substrate to
> divide the incoming beam into 64 beamlets and this lets them scan with
> 64 lines at once thereby cutting the scan time. They also use a
> combination of CCDs and NDDs as detectors. Can anybody comment on how
> effective this system is? Tom
>
>
>
> Thomas E. Phillips, Ph.D.
>
> Professor of Biological Sciences
>
> Director, Molecular Cytology Core
>
> 2 Tucker Hall
>
> Biological Sciences
>
> University of Missouri
>
> Columbia, MO 65211-7400
>
> 573-882-4712 (voice)
>
> 573-882-0123 (fax)
>
>
>
--
Alison J. North, Ph.D.,
Research Assistant Professor and
Director of the Bio-Imaging Resource Center,
The Rockefeller University,
1230 York Avenue,
New York,
NY 10065.
Tel: office ++ 212 327 7488
Tel: lab ++ 212 327 7486
Fax: ++ 212 327 7489