Re: Non Enzymatic Cell Harvesting Buffer

Posted by anh2006 on Apr 26, 2010; 4:32pm
URL: http://confocal-microscopy-list.275.s1.nabble.com/LaVision-TriM-multiphoton-microscope-tp4962077p4963514.html

I think you should test all reagents against your protein of interest - but I use TryplE Express for my receptor tyrosine kinases with success.

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-----Original Message-----
From: "B. Prabhakar Pandian" <[hidden email]>
Date: Mon, 26 Apr 2010 11:25:48
To: <[hidden email]>
Subject: Non Enzymatic Cell Harvesting Buffer

Hello,
          We are trying to harvest adherent cells following activation
with IL-1 for certain receptor upregulation. However, we  feel that the
trypsin-EDTA mixture used for harvesting is cleaving the receptors.
Can somebody suggest a proven non-enzymatic cell harvesting solution.


Thanks,

-Prabhakar