Re: Non Enzymatic Cell Harvesting Buffer

Posted by F Javier Díez Guerra on
URL: http://confocal-microscopy-list.275.s1.nabble.com/LaVision-TriM-multiphoton-microscope-tp4962077p4963696.html

El 26/04/2010 18:25, B. Prabhakar Pandian escribió:

> Hello,
>          We are trying to harvest adherent cells following activation
> with IL-1 for certain receptor upregulation. However, we  feel that
> the trypsin-EDTA mixture used for harvesting is cleaving the receptors.
> Can somebody suggest a proven non-enzymatic cell harvesting solution.
>
>
> Thanks,
>
> -Prabhakar

Hi,

Using cold PBS-EDTA (no trypsin), adherent cells detach although more
slowly.

Javier