Posted by
Haberman, Ann on
URL: http://confocal-microscopy-list.275.s1.nabble.com/LaVision-TriM-multiphoton-microscope-tp4962077p4970745.html
I've also had great experiences with LaVision. They are willing into incorporate custom ordered components, which they can produce with exquisite craftsmanship and elegant design. Very knowledgeable staff and most importantly, a desire to sell and support products that work.
I order a modified version of the TriM a couple of years ago, which now can be ordered as a standard model, so I can't comment on the 64 beam mode. This microscope is dedicated to a single beam of a two-photon laser and has a motorized control of the fill of the back aperture of the objective. It has been a total workhorse in an in vivo imaging facility that scans for hours at a time, day after day. The software doesn't have the same glitz and gloss that the major manufacturers offer, but the plus side is that it is easy to write macros that can be plugged into the software (if you are into that sort of thing).
They have a number of microscope in the US, but are a moderate sized company based in Germany. However, they respond quickly to my emails and phone calls and will stop by when they are in the area visiting other installed microscopes or conferences. I have even had a video conference once to assess a potential issue in real time, transcontinental, more quickly than a representative could have arrived.
Feel free to call with questions you might have,
Ann
>The TriM scope is a great system. We demo-ed it here in New York a couple of years ago. I was particularly impressed by the fact that they shipped it out here from Germany and then had it up and running well on live samples within about 2 hours. You can use it either in single-beam mode if you need to image deep, or in the 64-beam mode if you need to go fast but don't have such a requirement for depth. In the 64-beam mode I seem to remember we were limited to about 100-150 microns in depth, while in the regular mode we could go to around 300 microns or deeper, depending on the tissue. But the 64-beam mode certainly is fast, so although there are several excellent multiphoton options out in the field now - Olympus, Prairie, Zeiss etc. - I do think that the TriM has its own niche.
>A good thing about the company is that they are extremely knowledgeable and also very flexible about the system design - for example, they have integrated OPO lasers on their systems for some time now. The only downside as far as we were concerned is that it is a multiphoton-only system, and although I can see many advantages to that myself, some of my users were adamant about wanting visible lasers on our system as well. If you contact Sid Ragona, their US rep, (www.RagonaScientific.com) he can give you names of people in the US who are using the system.
>
>Best,
>Alison
>
>Phillips, Thomas E. wrote:
>>
>>I have been reading about the TriM multiphoton microscope from LaVision BioTec (
http://www.lavisionbiotec.com/en/microscopy-products/trim-scope/). They use a combination of mirrors and a beam splitting substrate to divide the incoming beam into 64 beamlets and this lets them scan with 64 lines at once thereby cutting the scan time. They also use a combination of CCDs and NDDs as detectors. Can anybody comment on how effective this system is? Tom
>>
>>
>>Thomas E. Phillips, Ph.D.
>>
>>Professor of Biological Sciences
>>
>>Director, Molecular Cytology Core
>>
>>2 Tucker Hall
>>
>>Biological Sciences
>>
>>University of Missouri
>>
>>Columbia, MO 65211-7400
>>
>>573-882-4712 (voice)
>>
>>573-882-0123 (fax)
>>
> Alison J. North, Ph.D.,
>Research Assistant Professor and Director of the Bio-Imaging Resource Center,
>The Rockefeller University,
>1230 York Avenue,
>New York,
>NY 10065.
>Tel: office ++ 212 327 7488
>Tel: lab ++ 212 327 7486
>Fax: ++ 212 327 7489
--
Ann Haberman, PhD
Department of Laboratory Medicine
Yale University School of Medicine
300 Cedar Street
TAC S541
New Haven, CT 06510
203-785-7349
203-785-5415 (fax)
[hidden email]