Re: EMCCD gain, photon scissors, bogeyman avoidance

Posted by Mark Cannell on
URL: http://confocal-microscopy-list.275.s1.nabble.com/EMCCD-gain-photon-scissors-bogeyman-avoidance-tp4972112p4972197.html

Karl Garsha wrote:
>
> To simplify and look at the big picture, let us contemplate, for the
> sake of discussion. What if, we first calculate our gain under non-EM
> conditions, using the non-EM read port. (by the way, the read noise
> here on a good EMCCD should be around 5-6 e- which is well in the
> range of a respectable fluorescence grade sony 285 interline).
Which EM chip has this read noise? I'd be interested in getting it for
our next EMCCD. FYI the charge amplifier should age much more slowly
than the EM register so you only need to calibrate when you need to
calibrate (which for most imaging is never, as I said).

Cheers