Two-photon imaging with low NA objectives?

Posted by stu_the_flat on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Two-photon-imaging-with-low-NA-objectives-tp4979404.html

Hello

In our lab, we have a two-photon microscope that has 10x NA 0.3 and a 40x NA
0.8 objective.

Recently some people in our lab have taken to using the 10x objective. I
calculate the point spread function is going to be 18.5µm! That is not
taking into account the tissue scattering effects (that I don’t know how to
calculate)

Can this set up just be thought of taking very broad optical section system
or are there other things to take into consideration?

Many thanks

Stuart