Re: EMCCD gain, photon scissors, bogeyman avoidance

Posted by Andreas Bruckbauer on
URL: http://confocal-microscopy-list.275.s1.nabble.com/EMCCD-gain-photon-scissors-bogeyman-avoidance-tp4972112p4984164.html

So i guess the question is if the mean variance method is working wel when using the  EMCCD chip witout the non-EM port, the problem might be aditional noise. I guess Karl at photometrics has tested this extensively. If you want to varify you probably need a calibrated light source.

best wishes

Andreas

-----Original Message-----
From: Mark Cannell <[hidden email]>
To: [hidden email]
Sent: Wed, 28 Apr 2010 2:47
Subject: Re: EMCCD gain, photon scissors, bogeyman avoidance

Karl Garsha wrote: 
> 
> To simplify and look at the big picture, let us contemplate, for the > sake of discussion. What if, we first calculate our gain under non-EM > conditions, using the non-EM read port. (by the way, the read noise > here on a good EMCCD should be around 5-6 e- which is well in the > range of a respectable fluorescence grade sony 285 interline). Which EM chip has this read noise? I'd be interested in getting it for our next EMCCD. FYI the charge amplifier should age much more slowly than the EM register so you only need to calibrate when you need to calibrate (which for most imaging is never, as I said). 
 
Cheers