Hi,
Sorry for late response here – traveling.
In our studies, Vectashield can initially
dim the dots and by as little as 3 days wipe out the signal entirely. (Prolong
Gold too.) Thus we strongly recommend our QMount mountant for optimal
viewing. See http://probes.invitrogen.com/media/pis/mp10336.pdf
for details.
Alternatively, for fixed or live samples
the photostabile Qdot nanocrystals can be imaged directly in solutions, like
PBS or media, though with some loss in initial signal compared to QMount. Coverslips
mounted in holders like our Attofluor chamber, or other coverslip bottomed
devices on inverted scopes work great as do dipping objectives on uprights. Not
great for archiving, but terrific for immediate, easy viewing.
Mike Ignatius
Molecular Probes/Life Technologies
From: Confocal
Microscopy List [mailto:[hidden email]] On Behalf Of Todorov, Ivan
Sent: Monday, April 26, 2010 9:00
PM
To:
[hidden email]
Subject: Re: Quantum dots mounting
media
Hi,
We are always using Vectashield from
Vector (no commercial interest) and never had problems with Qdots.
Best
Ivan
From: Confocal
Microscopy List on behalf of Kelly Rogers
Sent: Mon 4/26/2010 8:07 PM
To:
[hidden email]
Subject: Re: Quantum dots mounting
media
This is
correct. Some mounting media can not be used with Qdots.
Invitrogen sells a mounting media specifically for
Qdots called QMount.
Kelly
-no commercial interest
> As far as I remember only cytoseal can be
used. Others will kill the
> QD's.
>
> Diane
>
> -----Original Message-----
> From: Confocal Microscopy List
> [[hidden email]]On
Behalf Of Maria Calvo
> Sent: Monday, April 26, 2010 2:30 PM
> To: [hidden email]
> Subject: Quantum dots mounting media
>
>
> Dear all,
>
> Can anyone tell me which is the optimal
mounting media (composition) for
> quantum dots labelled samples? Which
reactives should be avoided during
> the immunochemistry with QD?
>
> Thank you for your help!
>
> Maria Calvo
>
> --
> Dra. Maria Calvo
>
> Microscòpia Confocal. Unitat de
Microscòpia
> Serveis Cientificotècnics-Campus
Casanova
> Facultat de Medicina
> Universitat de Barcelona. IDIBAPS
> Casanova 143
> Barcelona 08036
>
> Tel: 34 934037159
> Fax: 34 934044408
> E-mail: [hidden email]
>
Kelly Rogers PhD
The Walter & Eliza Hall Institute
1G Royal Parade, Parkville
Victoria 3052, Australia
ph: +61_3_9345 2450
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