Tracking cells automatically during a long time-lapse experimnet

Posted by Romin, Yevgeniy/Sloan Kettering Institute on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Tracking-cells-automatically-during-a-long-time-lapse-experimnet-tp5009517.html

Dear List

 

I have another question about live imaging, since all of you have been so helpful with your responses before.  We are planning on running an experiment where we want to follow a cell that will be moving quite a lot over an extended period of time.  We need to somehow keep this cell in the field of view throughout the whole experiment.  The two possibilities that come to mind are either taking a relatively large tilescan at every timepoint and hope that the cell will not go past the area, or writing a lengthy and complex journal in a program like Metamorph in order to detect the cells movement within the field and move the stage with it over time.  Has anyone done a similar experiment?  Are there any commercial systems available that are able to do this?

 

Thanks very much to everybody in advance,

 

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Yevgeniy Romin

 

Digital Microscopist

Memorial Sloan-Kettering Cancer Center

Molecular Cytology Core Facility

1275 York Ave. Box 333

New York, NY 10065

Tel.[phone]

Fax. [phone]

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