Re: Tracking cells automatically during a long time-lapse experimnet

Posted by Martin Wessendorf-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Tracking-cells-automatically-during-a-long-time-lapse-experimnet-tp5009517p5009613.html

Dear Yevgeniy--

If I remember correctly, Keith Lidke in the Physics Dept at University
of New Mexico has written a MatLab script that can move the stage to
keep a particle in the middle of a field of view.  Not sure whether or
not that would work with a cell but you might want to contact him and check.

Good luck--

Martin Wessendorf

Yevgeniy Romin wrote:

> Dear List
>
>  
>
> I have another question about live imaging, since all of you have been
> so helpful with your responses before.  We are planning on running an
> experiment where we want to follow a cell that will be moving quite a
> lot over an extended period of time.  We need to somehow keep this cell
> in the field of view throughout the whole experiment.  The two
> possibilities that come to mind are either taking a relatively large
> tilescan at every timepoint and hope that the cell will not go past the
> area, or writing a lengthy and complex journal in a program like
> Metamorph in order to detect the cells movement within the field and
> move the stage with it over time.  Has anyone done a similar
> experiment?  Are there any commercial systems available that are able to
> do this?
>
>  
>
> Thanks very much to everybody in advance,
>
>  
>
> ---------------------------------------------------
>
> *Yevgeniy Romin*

--
Martin Wessendorf, Ph.D.                   office: [phone]
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