Re: Tracking cells automatically during a long time-lapse experimnet

Posted by lechristophe on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Tracking-cells-automatically-during-a-long-time-lapse-experimnet-tp5009517p5009791.html

Dear Yevgeniy,

You should have a look at J. Ellenberg's paper that has done automatic
cell tracking using Zeiss LSM software to keep a cell at the center of
the field of view :

Rabut G, Ellenberg J. Automatic real-time three-dimensional cell tracking by
fluorescence microscopy. J Microsc. 2004 Nov;216(Pt 2):131-7. PubMed PMID:
15516224.
http://www3.interscience.wiley.com/journal/118757070/abstract


Christophe


On Wed, May 5, 2010 at 17:55, Martin Wessendorf <[hidden email]> wrote:

>
> Dear Yevgeniy--
>
> If I remember correctly, Keith Lidke in the Physics Dept at University of New Mexico has written a MatLab script that can move the stage to keep a particle in the middle of a field of view.  Not sure whether or not that would work with a cell but you might want to contact him and check.
>
> Good luck--
>
> Martin Wessendorf
>
> Yevgeniy Romin wrote:
>>
>> Dear List
>>
>>
>> I have another question about live imaging, since all of you have been so helpful with your responses before.  We are planning on running an experiment where we want to follow a cell that will be moving quite a lot over an extended period of time.  We need to somehow keep this cell in the field of view throughout the whole experiment.  The two possibilities that come to mind are either taking a relatively large tilescan at every timepoint and hope that the cell will not go past the area, or writing a lengthy and complex journal in a program like Metamorph in order to detect the cells movement within the field and move the stage with it over time.  Has anyone done a similar experiment?  Are there any commercial systems available that are able to do this?
>>
>>
>> Thanks very much to everybody in advance,
>>
>>
>> ---------------------------------------------------
>>
>> *Yevgeniy Romin*
>
> --
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