Re: Tracking cells automatically during a long time-lapse experimnet *COMMERCIAL RESPONSE*

Posted by Donnelly, Tom on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Tracking-cells-automatically-during-a-long-time-lapse-experimnet-tp5009517p5024232.html

Nico;

 

Since the release of the RT version about five years ago all DeltaVision systems have been able to track cell movement and return the target to the center of the field of view.

 

The user selects a ROI and chooses to track either center of mass or center of intensity.  The user then selects a threshold distance.  When the ROI reaches the threshold the XYZ stage returns the target to the center of the field of view and records new X-Y coordinates.

 

This process works equally well for single and multi-point visiting experiments.

 

Cheers,

 

Tom Donnelly

www.appliedprecision.com

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From: Nico Stuurman [mailto:[hidden email]]
Sent: Saturday, May 08, 2010 9:28 AM
To: [hidden email]
Subject: Re: Tracking cells automatically during a long time-lapse experimnet

I have another question about live imaging, since all of you have been so helpful with your responses before.  We are planning on running an experiment where we want to follow a cell that will be moving quite a lot over an extended period of time.  We need to somehow keep this cell in the field of view throughout the whole experiment.  The two possibilities that come to mind are either taking a relatively large tilescan at every timepoint and hope that the cell will not go past the area, or writing a lengthy and complex journal in a program like Metamorph in order to detect the cells movement within the field and move the stage with it over time.  Has anyone done a similar experiment?  Are there any commercial systems available that are able to do this?

The non-commercial (free) Micro-Manager software ( http://micro-manager.org ) includes the "Tracker" plugin that keeps moving cells centered in the image by moving the stage to correct for cell movement.  It was build and tested to track moving keratocytes.  As it uses cross-correlation between images it is likely to work with most other moving objects as well.  Micro-Manager works with most microscope hardware components.

Best,

Nico

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