Re: Tracking cells automatically during a long time-lapse experimnet

Posted by Cameron Nowell on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Tracking-cells-automatically-during-a-long-time-lapse-experimnet-tp5009517p5024812.html

Hi Nico,
 
I would have thought that the journal for MetaMorph woudln't be that complex. All you need to do is get the X,Y co-ordinates variable of the object you are interested, measure the diference in them each time point and add (or subtract) that difference from the stage X,Y co-ordinates variable. Of course this could get a bit tricky if there were too many cells in each field.
 
Feel free to contact me off list if you want any help putting the journal together.
 
 
Cheers
 
 
Cam
 
 
 
Cameron J. Nowell
Microscpy Manager
Central Resource for Advanced Microscopy
Ludwig Insttue for Cancer Research
PO Box 2008
Royal Melbourne Hospital
Victoria, 3050
AUSTRALIA
 
Office: [phone]
Mobile: [phone]
Fax: [phone]
 
http://www.ludwig.edu.au/branch/research/platform/microscopy.htm
 

________________________________

From: Confocal Microscopy List on behalf of Nico Stuurman
Sent: Sun 9/05/2010 2:27 AM
To: [hidden email]
Subject: Re: Tracking cells automatically during a long time-lapse experimnet



       
        I have another question about live imaging, since all of you have been so helpful with your responses before.  We are planning on running an experiment where we want to follow a cell that will be moving quite a lot over an extended period of time.  We need to somehow keep this cell in the field of view throughout the whole experiment.  The two possibilities that come to mind are either taking a relatively large tilescan at every timepoint and hope that the cell will not go past the area, or writing a lengthy and complex journal in a program like Metamorph in order to detect the cells movement within the field and move the stage with it over time.  Has anyone done a similar experiment?  Are there any commercial systems available that are able to do this?


The non-commercial (free) Micro-Manager software ( http://micro-manager.org <http://micro-manager.org/>  ) includes the "Tracker" plugin that keeps moving cells centered in the image by moving the stage to correct for cell movement.  It was build and tested to track moving keratocytes.  As it uses cross-correlation between images it is likely to work with most other moving objects as well.  Micro-Manager works with most microscope hardware components.

Best,

Nico